抗坏血酸测定试剂盒

测定方法     比色法（570nm）或荧光法（530nm/590nm）定量测定抗坏血酸（维生素C）的浓度。

产品说明   抗坏血酸（其L-对映异构体便是人们所熟知的维生素C）是生物体中一种重要的抗氧化成分，也是食品及其他工艺过程中的添加剂。通过与活性氧发生反应，抗坏血酸可保护细胞免受氧化损伤。本公司的产品为测定抗坏血酸提供了一种简便、直接、高通量的检测方法。该方法利用抗坏血酸与抗坏血酸氧化酶反应生成H₂O₂。H₂O₂与一种特殊的显色剂反应形成一种粉红色产物，其吸光强度（570nm）或荧光强度（530/585nm） 直接与样品中的抗坏血酸浓度成正比。

产品特点     线性检测范围：比色法6-1000 mM，荧光法 1-100mM 抗坏血酸。

适用范围  检测生物样品如血清、血浆、尿液、唾液、乳汁、组织和细胞培养物的抗坏血酸浓度。药物鉴定/药理学：药物对抗坏血酸代谢的影响。

贮藏条件     试剂盒应冷冻运输，所有试剂均于-20°C保存。签收后3个月内有效。

DEscriptION  Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. Our method provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.

KEY FEATURES  Use 20 μL samples. Linear detection range: colorimetric assay 6 to 1000 μM, fluorimetric assay 1 to 100 μM ascorbic acid.

APPLICATIONS  Assays: ascorbic acid in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture. Drug Discovery/Pharma-cology: effects of drugs on ascorbic acid metabolism.

STORAGE CONDITIONS  The kit is shipped on ice. Store all components at -20°C. Shelf life of three months after receipt.

编号：EASC-048, EASC-100

测试：48T, 100T