产品编号:ES-SJ-M0019
CAS号:9001-51-8
中文名称:己糖激酶
英文名称:HK
其他中文名称:己糖磷酸激酶
其他英文名称:Hexokinase from Saccharomyces cerevisiae;ATP:D-Hexose-6-phosphotransferase;Hexokinase from yeast
包 装:1克
级别:BR
类型:Type Ⅲ
分子量:~ 54 kDa (monomer);~110 kDa (dimer)
活力:≥25units/mg protein (biuret)
活力定义:One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25℃
Optimal pH:7.5~9.0
Protein by biuret:≥60%
Extinction Coefficient:E1% = 8.85 (PI) and 9.47 (PII) at 280 nm
Foreign activity:glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%
性状:白色粉末或鳞状粉末。酿酒酵母提取。催化剂(Activators):Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds;抑制剂(Inhibitors):sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
用途:生化研究。为催化己糖6位上由ATP使之产生磷酸化反应的酶。对α-D葡萄糖的亲和性很高(Km=1×10-5M),对其他的己糖也可进行磷酸化。此反应的平衡向右偏移。△G°’=4.0千卡。该酶可被Mg2 、 K 所活化,但可被HS试剂阻抑。
保存:-20℃