产品品名:D-木糖检测试剂盒
英文品名:D-Xylose Assay Kit
规格型号:100 assays (manual) / 1000 assays (microplate) / 1300 assays (auto-analyser)
Novel method for the specific, convenient and rapid assay of D-xylosein plant extracts, culture media/supernatants and other materials.
Suitable for manual, auto-analyser and microplate formats.
INTRODUCTION:
In nature, D-xylose occurs mainly in the polysaccharide form as xylan,
arabinoxylan, glucuronoarabinoxylan, xyloglucan and xylogalacturonan.
Mixed linkage D-xylans are also found in certain seaweed species and a
similar polysaccharide is thought to make up the backbone of psyllium
gum. Free D-xylose is found in guava, pears, blackberries, loganberries,
raspberries, aloe vera gel, kelp, echinacea, boswellia, broccoli, spinach,
eggplant, peas, green beans, okra, cabbage and corn. In humans, D-xylose
is used in an absorption test to help diagnose problems that prevent the
small intestine from absorbing nutrients, vitamins and minerals in food.
D-Xylose is normally easily absorbed by the intestine. When problems
with absorption occur, D-xylose is not absorbed and blood and urine
levels are low. A D-xylose test can help to determine the cause of a
child’s failure to gain weight, especially when the child seems to be eating
enough food. If in a polysaccharide, the ratio of D-xylose to other sugars
etc. is known, then the amount of the polysaccharide can be quantitated
from this knowledge plus the determined concentration of D-xylose in
an acid hydrolysate. Xylans are a major portion of the polysaccharides
that could potentially be hydrolysed to fermentable sugar for biofuel
production.
KITS:
Kits suitable for performing 100 assays in manual format (or 1300
assays in auto-analyser format or 1000 assays in microplate format) are
available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (45 mL, pH 7.5) plus sodium azide (0.02% w/v)
as a preservative.
Stable for 大于 2 years at 4°C.
Bottle 2: (x2) NAD+ plus ATP.
Freeze dried powder.
Stable for 大于 5 years at -20°C.
Bottle 3: Hexokinase suspension (2.2 mL).
Stable for 大于 2 years at 4°C.
Bottle 4: XDH/XMR solution (5.6 mL).
Stable for 大于 2 years at -20°C.
Bottle 5: D-Xylose standard solution (5 mL, 0.25 mg/mL).
Stable for 大于 2 years at 4°C.
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