海南大学食品科学与工程学院的Huihui Sun、Xiaoxiang Peng、Chuan Li等人建立了一种可靠的反相液相色谱法测定羰基化合物，并验证了2,4-癸二烯醛作为目标醛的可行性。此外，还评估了2,4-癸二烯醛作为脂质氧化标记物的可能性。最佳的样品预处理方法是用2 mL乙腈萃取3次，然后在40 ℃温度下衍生30 min。该方法线性、灵敏、准确，检测和定量限分别为15和50 nmol/L，对油样中的2,4-癸二烯醛具有良好的平均回收率。在被测食用油中，180 ℃加热期间，2,4-癸二烯醛的含量比其他醛类（包括其中一种特征性醛类）上升得更快。
此外，在3 种温度（120、150、180 °C）下加热8 h的向日葵和玉米油中，2,4-癸二烯醛含量与其他氧化指数（茴香胺值的R2=0.858至0.984，总氧化值的R2=0.876至0.986）之间存在良好的线性关系，表明2,4-癸二烯醛可以预测油的氧化。
Determination of 2,4‐decadienal in edible oils using reversed‐phase liquid chromatography and its application as an alternative indicator of lipid oxidation
Huihui Sun Xiaoxiang Peng Chuan Li Wei‐Min Zhang Jun Cao
College of Food Science and Engineering, Hainan University, Haikou, Hainan, 570228 China
Abstract
As a major product of linoleic acid‐rich oils, 2,4‐decadienal has unique reactivity that may be potentially toxic to human body. In this study, a reliable reversed‐phase liquid chromatography method for the determination of carbonyls was developed, and 2,4‐decadienal as the target aldehyde was validated. Furthermore, the possibility of 2,4‐decadienal as a lipid oxidation marker was evaluated. The optimal sample pretreatment method was extraction by 2 mL of acetonitrile three times, followed by derivatization at 40 °C for 30 min. The method was linear, sensitive, and accurate with detection and quantification limits of 15 and 50 nmol/L, respectively, and had good average recoveries for 2,4‐decadienal in oil samples. In tested edible oils, during heating at 180 °C, the level of 2,4‐decadienal rose faster than other aldehydes, including one of the characteristic aldehydes, hexanal. Moreover, good linear relationships between the 2,4‐decadienal content and other oxidation indices (R2=0.858 to 0.984 for the anisidine value; R2=0.876 to 0.986 for the total oxidation value) were observed in sunflower and corn oils under 8 hr heating at three temperatures (120, 150, and 180 °C), indicating that 2,4‐decadienal can predict the oxidation of oil.
