从菌落的结果来看，超声波对大肠杆菌和金黄色葡萄球菌均有不可逆转的作用，没有亚致死性损伤。该研究的结果还表明，一部分细菌亚群细胞内成分（例如DNA和酶）遭受严重损伤，但具有完整的细胞膜。研究者推测超声波对微生物的灭活作用可能不仅仅是完成细胞外部的破坏。那些未进入超声空化有效区域的微生物细胞可能被注射超声产生的自由基，并且经历了具有完整外部结构的内部损伤，并且与超声波场接近的其他细胞将被完全破坏成高强度机械力量形成的碎片。这些发现试图为超声波对微生物的灭活机制提供延伸。
文章摘要/abstract
Multiple action sites of ultrasound on Escherichia coli and Staphylococcus aureus
Xinyu Liao a,b,c, Jiao Li a,b, Yuanjie Suoa,b, Shiguo Chena,b, Xingqian Ye a,b,Donghong Liu a,b,c, Tian Ding a,b,c,?
（a. Department of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou,Zhejiang 310058, China
b. Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, Zhejiang 310058, China
c. Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China）
Received 24 November 2017; received in revised form 7 January 2018; accepted 22 January 2018
Available online 15 February 2018
Abstract:
Ultrasound, is thought to a potential non-thermal sterilization technology in food industry. However, the exact mechanisms underlying microbial inactivation by ultrasound still remain obscure. In this study, the action modes of ultrasound on both Gram-negative and Gram-positive microorganisms were estimated. From colony results, ultrasound acted as an irreversible effect on both Eshcerichia coli and Staphylococcus aureus without sublethal injury. The result in this study also showed that a proportion of bacteria subpopulation suffered from serious damage of intracellular components (e.g. DNA and enzymes) but with intact cell envelopes. We speculated that the inactivated effects of ultrasound on microbes might more than simply completed disruption of cell exteriors. Those microbial cells who had not enter the valid area of ultrasonic cavitation might be injected with free radicals produced by ultrasound and experienced interior injury with intact exterior structure, and others who were in close proximity to the ultrasonic wave field would be immediately and completely disrupted into debris by high power mechanic forces. These findings here try to provide extension for the inactivation mechanisms of ultrasound on microorganisms.
