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High purity reagent for assay of D-glucose in cereal extracts and for use in combination with other Megazyme kits. INTRODUCTION: D-Glucose can be conveniently measured in body fluids using commercially available kits based on the glucose oxidase/peroxidase or on the hexokinase/G6P-DH enzymic procedures. However,D-glucose in plant extracts usually occurs together with maltose, maltosaccharides, starch, sucrose and/or β-linked gluco-oligosaccharides. Consequently, more stringent requirements are placed on the purity of the assay reagents. The reagents must be essentially devoid of starch degrading enzymes, sucrose degrading enzymes and β-glucosidase, as these can lead to either an over-estimation or an under-estimation of free D-glucose present in the extract or derived by specific enzymic degradation of D-glucose containing oligosaccharides or polysaccharides (e.g. barley β-glucan). Most commercially available D-glucose kits based on the glucose oxidase/peroxidase reaction contain reagents which are not sufficiently pure.The Megazyme D-Glucose (glucose oxidase/peroxidase; GOPOD) Assay Kit employs high purity glucose oxidase and peroxidase and can be used with confidence for the specific measurement of D-glucose in extracts of plant materials or foods. The colour which forms is stable at room temperature for at least two hours after development. KITS: Kits suitable for performing 660 assays (3 mL per assay) are available from Megazyme. The kits contain the full assay method plus:Bottle 1: (x 2) GOPOD Reagent Buffer. Buffer (48 mL, pH 7.4), p-hydroxybenzoic acid and sodium azide (0.4% w/v).Stable for > 4 years at 4°C.Bottle 2: (x 2) GOPOD Reagent Enzymes. Glucose oxidaseplus peroxidaseand 4-aminoantipyrine. Freeze-dried powder.Stable for > 5 years at -20°C.Bottle 3: D-Glucose standard solution (5 mL, 1.0 mg/mL) in 0.2% (w/v) benzoic acid.Stable for > 5 years at room temperature.